Innovations in imaging system design: The proposed NAM method provides a robust way to transform the problem of high-throughput microscopy from one that is tied to physical limitations of the optics to one that is computationally solvable.
This study leads to interesting findings such as a hierarchy of neutrophil response to chemical signals, the involvement of an enzyme in regulating neutrophils to decide which signal to follow, and the involvement of endothelial cells in regulating neutrophil chemotaxis through surface marker expression.
To this end, we present a lensless microscopy solution termed ePetri-dish. Suggested Citation Kim, Donghyuk.
Mesoporous silica nanoparticles are used as the model nanoparticles and human endothelial cells and platelets are used as the model cell types to mimic human blood vessels. We present two novel optical structure designs: Imager modification is an emerging technique that performs pre-detection light field manipulation.
In both cases, the use of thermally sensitive resonant optical devices is critical for both energy-efficiency and compactness of the optical components. Finally, we utilize these pieces to demonstrate a processor chip which uses on-chip photonic interconnects to optically communicate to distant off-chip main memory.
The first design strategy involves the active control of the illumination sources. By using a model of chemotaxis, chemically directed cellular migration, the dynamics of cellular behaviors under multiple chemical signals Chapter 3 and in the context of cell-cell interactions Chapter 4 were quantitatively studied using a microfluidic platform.
Based on such a light path, this thesis presents several new microscopy imaging techniques from three aspects: In a typical microscopy imaging platform, the light path can be generalized to the following steps: The active control of illumination sources can also be adapted for chip-scale microscopy imaging.
Monday, May 4, - 9: The third accessing point in design considerations is the image sensor. We show that the NAM method is capable of providing two orders of magnitude higher throughput for most existing bright-field microscopes without involving any mechanical scanning.
The target cells used in these chapters are neutrophils, the most abundant and motile leukocyte in humans. Well-designed microfluidic approaches can reveal both biophysical and biochemical aspects of human in vivo systems with quick and temporally, spatially, and chemically resolved analyses of in vitro assays.
These structures can be directly incorporated onto optical sensors to accomplish pre-detection background suppression and wavefront sensing. The microfluidic platform establishes a stable and dynamic gradient of chemicals across a cell culture chamber and enables the quantitative investigation of cellular migration in the presence of multiple chemical signals.
The developed microfluidic sensor platforms are equipped with gold-based SERS substrates that enable highly sensitive chemical fingerprinting, and the microfluidic device enables serial dilution of introduced analyte solution that terminates in five discrete sensing elements.
Compared to traditional cytotoxicity assays performed under static conditions, mesoporous silica nanoparticles show higher and shear stress-dependent toxicity to endothelial cells under flow conditions.
In parallel to above mentioned biochemical studies with in vivo mimetic microfluidic platforms, this thesis also aims to achieve microfluidics-based analytical platforms with powerful chemical identification capability.
The aim of this thesis is to enable the study of cellular behaviors in physiologically relevant environments using microfluidics. In addition, this study also reveals the impact of neutrophil activation in neutrophil chemotaxis, providing insights into the potential of neutrophil chemotaxis regulation for therapeutic purposes.
Thus far, however, both electronic-photonic process compatibility and the difficulty of working with thermally sensitive optical devices have limited the achievable system complexity.On-Chip Memory Architecture Exploration of Embedded System on Chip A Thesis Submitted for the Degree of Doctor of Philosophy in the Faculty of Engineering.
This handout describes what a thesis statement is, how thesis statements work in your writing, and how you can discover or refine one for your draft.
System-on-Chip Modules Formale Veriﬁkation von ﬁrmwarebasierten System-on-Chip-Modulen Vom Fachbereich Elektrotechnik und Informationstechnik der Technischen Universität Kaiserslautern This thesis is the result of several years of intense and interesting research at the Elec.
System on Chip: It is an integrated circuit which integrates all the components like processor. Multiple slave devices are allowed with individual slave select (chip select) lines. Quad SPI: It is an advanced version of the SPI.
This thesis covers the design and analysis of the on-chip network and its integration with the SCMP system. The result of these efforts is a framework for analyzing on-chip.
multiplexed polymer microfluidic chip is demonstrated as a simple and robust method with uniform spotting volume and MS signal. This automatic contact spotting system.Download